Direct detection of endogenous hydroxyl radical production in cultured adult cardiomyocytes during anoxia and reoxygenation. Is the hydroxyl radical really the most damaging radical species?
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چکیده
منابع مشابه
Radical Production in Cultured Adult Cardiomyocytes During Anoxia
Isolated adult rat cardiac myocytes were subjected to anoxia and substrate deprivation for 15, 30, 60, 90, and 120 minutes and reoxygenation for 120 seconds. The supernatant and cell extract were analyzed for hydroxyl radicals ( OH) with high-performance liquid chromatography using salicylate as a trapping agent. The production of intracellular H202 as a possible precursor of * OH was also docu...
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Under physiological and pathological conditions, the reactive oxygen species (ROS) are formed from both exogenous and endogenous sources. ROS are implicated in a wide number of disease processes, including atherosclerosis, autoimmune disorders, rheumatoid arthritis, neuronal degeneration, cardiovascular diseases and cancer. Among the reactive oxygen species produced in living cells, hydroxyl ra...
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The chemistry of oxidative deposition of iron(III) in ferritin and apoferritin is poorly understood. This study was undertaken to look for radicals formed as the hydrous ferric oxide core is developed from Fe(II) and O2. Radicals were observed indirectly by using the spin-trapping reagent N-tert-butyl-alpha-phenylnitrone (PBN) at room temperature and directly by measuring ESR spectra of frozen ...
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Reperfusion of blood flow to an ischemic myocardium is imperative to survival; ironically, it may also manifest several pathophysiological conditions. The most important of these are reperfusion arrhythmias and tissue injury and/or death. The mechanisms involved in reperfusion arrhythmias remain to be fully elucidated; however, increasing evidence indicates that reperfusion-induced arrhythmias ...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 1993
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.72.4.725